Synthetic Milk

Assessment of synthetic milk exposure to children of selected population in Uttar Pradesh, India Shuchi R. Bhatt1, Dr. Anita Singh2 and S. M. Bhatt3 (Abstract) The present study was carried out keeping in view the recently emerging concern of the adulteration of the natural milk with the synthetic milk. Synthetic milk is prepared by emulsifying vegetable oils with appropriate amount of detergent and urea. Samples of the natural milk with synthetic milk were analyzed for concentration of urea and detergent and their effect is assayed through survey in different population in Uttar Pradesh, India.Children’s had different range of intake of milk. Children of age group 1-5 years consumes about 50-250 mg of milk daily, while of age group 6-18 years of children consumes about 250-1000 ml milk/day and children’s of age group 19-22 consumes milk about 500-1000 ml milk /day. Addition of synthetic milk is on large scale in Meerut district of U. P. and mostly urea in such milk cre ating huge problem of headache, eyesight and diarrhea in children. Keywords: Milk adulteration; Synthetic milk, detection, urea 1.Research Scholar, Faculty of H. Sc, Sri. A. K. A. P. G. Collage Varanasi. 2. Head, Department of Food & Nutrition, Sri. Agrasen Kanya Autonomous P. G. College. Parmanandpur, Varanasi. 3. School of Biochemical Engineering, Institule of Technology B. H. U. Varanasi. Corresponding Author Email: [email  protected] co. in 1 Introduction Liquid milk is an essential nutritional food for infants as well as the aged. Adulteration of natural milk with a chemically synthesized milky liquid (synthetic milk) is a matter of serious concern.The dairy industry employs various checks these tests commonly include determination of fat and total solids by chemical or physical analyses; estimation of sediment by forcing milk through filter pads and noting the residue left; determination of Bacterial count and determination of freezing point etc. (Dean, 1985; Fox, 1992). How ever, most of these measurements are expensive and time consuming like measurement of conductance (Mebrook & Petty 2003 a and b; Willard et al 2003). Synthetic milk is an excellent imitation of natural milk.Milk fat is mimicked by vegetable oil; the nitrogen component in milk is mimicked by urea; detergents are added to make it frothy. This mixture is so expertly prepared that the specific gravity of the concocted milk is the same as natural buffalo milk. This mixture is then mixed with natural milk in varying proportions. Such milk can be processed into ‘‘value added’’ products which bring in a bigger profit. A recent Indian Council of Medical research (ICMR) report has suggested that such adulterated items have a cancerous effect on the human system and can lead to gradual impairment of the body.Milk is a complex mixture of water, lactose, fat, protein, minerals, and vitamins distributed throughout colloidal and soluble phases. Although the cow is the pri ncipal source of milk for human consumption in the United States and many other parts of the world (DePeters 1992). In India most milk is 2 obtained from the buffalo. The composition of milk from buffalo is water (82. 14%), fat (7. 44%), protein (4. 78%), lactose (4. 8%) and ash (0. 83%) (Rangappa & Achary, 1973).On average milk has optimum level of urea and other chemicals but due to habit of making huge profit there is on going malpractice of using synthetic milk in the original milk. Therefore there is need to work out regularly in different region of India to expose such malpractices and to check such malpractices adapted ignoring health concerns of people. The effect of urea on animal reproductive system has been carried out by Ropstad etal 1987. They reported that reproduction system and reproductive cycle in animals get badly affected by high concentration of urea.This work is carried out to expose and to increase the awareness among people to decline such malpractices adapte d, since it is difficult to detect such harmful adulterants in dangerous amount in household. One quick method describe in the article is to check the pH of the milk by pH paper (red turns blue) that is available freely and can’t be adjusted quickly at large scale. Material and method Survey Preschool (1-5 years) and school going (6-18 years) children in urban and rural area from different district of U. P. had been surveyed.A total of 365 household were surveyed in which 70 children’s were of age group 1-5 years, 150 children’s were of age group 6-18 years, and 145 children’s were of age group 19-22 years. Since it was household survey, the homemaker of the household was interviewed for intake of milk. The region of study selected was Varanasi, Bulandsahar, Meerut and Mathura. Thus, 365 individuals in urban area and 365 in rural area were surveyed. The milk were 3 collected from the household and tested for presence of urea and detergent. The frequency m ethod was employed for calculation.The survey includes consumption of milk/day, their effect on health such as headache, eyesight problem, and diarrhea. Also, 160 sample of the milk were collected from different local market, vendors, and stalls of Bulandsahar, Mathura, Meerut, and Varanasi. Qualitative analysis for urea detection Qualitative analysis for urea detection were done by the method describe below 1. Took 5 ml of milk in a test tube 2. Added 20 mg of Soya bean powder & 2 drops of 0. 5 % aqueous solution of Bromothymol blue. 3. Mixed the sample well. 4. The development of Blue colour after 10 min indicates the addition of urea the sample. 5.For confirmatory test was done by adding five ml of milk with 5 ml paradimethyl amino benzaldehyde (16%). If the solution turns yellow in colour, then the given sample of milk is added with urea. Quantitative analysis for urea detection A quantitative estimate of urea was carried out by preparing standard of urea and their pH variation and by utilizing Urease enzyme. For this we took 5 ml of milk in a test tube and added 0. 2 ml of urease (20 mg / ml) and centrifuged it well at room temperature. Now added 0. 1 ml of bromothymol blue solution (0. 5%). The appearance of blue colour after 10-15 min indicates the adulteration milk with urea.Urease can be obtained by adding 4 soybean slurry (source of urease prepared by soaking soybeans overnight or for 12 hours in water and grinding to prepare a slurry). (Dean J. A 1985) The OD of sample was taken in UV-Vis Spectrophotometer (ELICO double beam) at 420 nm. And pH was measured by pH meter using buffer sample set at pH 4. In the presence of urea the red litmus paper turns blue. Urea Standard Solution was prepared by following method – (a) Stock solution concentration was 5 mg / ml. Dissolved 50 gm reagent grade urea in water and dilute to 1 litre with water. b) Working solution was prepared by Pipetting 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 ml stock solution into 250 ml volumetric flask and dilute to volume with phosphate buffer. (c) Reference solution – standard solution was containing 1. 0 mg urea / 5 ml as reference standard. Figure caption Fig. 1 showing variation of pH with different concentration of urea Fig. 2 showing variation of urea and detergent in different region of Uttar Pradesh India Fig. 3 showing children affected by synthetic milk in different age group (a) Headache; (b) Eyesight ; and (c) Diarrhea. 5 Figures Urea 5 4 Urea % (w/v) 2 1 0 1 2 3 4 5 6 pH Fig. 1 showing variation of pH with different concentration of urea 60 Urban Rural % of Urea and detergent adultrants 50 40 30 20 10 0 Bulandsahar Mathura Meerut Varanasi Fig. 2 showing variation of urea and detergent in different region of Uttar Pradesh India 6 Headache 40 35 30 Urban Rural 60 50 Eye sight problem Urban Rural % of sample 25 20 15 10 5 0 1-5 6-18 19-22 % of sample 40 30 20 10 0 1-5 6-18 19-22 age group Age group (a) (b) % of children affected by problem of Diarrhea 60 50 Urban Rural % of total sample 40 30 20 10 0 1-5 6-18 19-22 Age group (c) Fig. showing children affected by synthetic milk in different age group (a) Headache; (b) Eyesight ; and (c) Diarrhea. 7 Tables Table-1 showing different constituents of milk CONSTITUENTS Water Lipid in emulion phase ( mixture of mixed triglecerides) Phospolipids ( lacithine, Cephaline, ) STEROLS carotenoids vit. A, D. E. K Protiens (Casein) B- Lactoglobulin Lactoalbumin Albumin, Psuedoglobulin etc Enzymes ( Catalase, Peroxidase, Amylase, Lipase, Protease) DESSOLVED MATERIALS Carbohydraetes Lactose Glocose ORGANIC AND INORGANIC ION & SALTS Calcium Phosphate Citrate Chloride, Sodium, Pottasium, Magnesium etc.Water soluble Vitamins Thiamin APPROX.. CONCENTRATION / LIT 860 TO 880 gms 30-50gms 0. 30 gms 0. 10 gms 25 gms 3 gms 0. 7gms 40-50 gms 50 mgs 1. 25 gms 2. 10 gms 2. 0 gms 1. 00 gms 0. 4 mg 1. 5 mg 8 Riboflavin Niacin Pyridoxin Pentothenic Acid Biotin Folic acid Chline (Total) Vit B12 inosi tol Ascorbic acid Nitrogenous material Ammonia Amino Acids Urea Creatine and Createnine Uric acid Gases Carbon dio Oxide Oxygen Nitrogen 0. 2 -1. 2 mg 0. 7mg 3. 0 mg 50 mg 1. 0 mg 150 mg 7. 0 mg 180 mg 20 gm 2-12 mg 3. 5 mg 100 mg 15 mg 7 mg Milk exposed to air 15 mg 7. mg 15 mg Copper, Iron, Rb, Trace element Li, Ba, Mn, Al, Zn, B, Co, I Occasionally Present Mc, Cr, Ag, Sn, Ti, 9 Table 2. Average Composition of milk Item Water Lactose Fat True Protein Crude Protein Casein Ash Other Average milk composition % 87. 00 4. 90 3. 70 3. 00 3. 10 2. 60 . 80 . 50 Table -3 showing average milk consumed by different age group Mean of Milk age (years) 1-5 years 6-18 years 19-22 years children consumed 70 150 145 156. 428 SD range 53. 78 50-250 500 274. 77 250-1000 800 224. 22 500-1000 10Table-4 Effect of urea on different age groups in urban area No. of Age group 1-5 years 6-18 19-22 children Headache Eye problem 70 150 145 nil 42 56 8 86 76 Digestion /diarrhea 32 86 76 normal 38 60 69 Table-5 Effect of urea on different age groups in rural area (365 samples) No. of Age group 1-5 years 6-18 19-22 children Headache Eye problem 70 150 145 nil 6 16 2 25 18 Digestion /diarrhea 16 25 18 normal 54 125 127 Table-6 Sample tested in rural area (sample tested 160) No. of sample 40 38 32 50 City >5% urea 5% urea

Gigantopithecus - Facts and Figures

Gigantopithecus - Facts and Figures Name: Gigantopithecus (Greek for giant ape); prounced jie-GAN-toe-pith-ECK-us Habitat: Woodlands of Asia Historical Epoch: Miocene-Pleistocene (six million to 200,000 years ago) Size and Weight: Up to nine feet tall and 1,000 pounds Diet: Probably omnivorous Distinguishing Characteristics: Large size; large, flat molars; four-footed posture About Gigantopithecus The literal 1,000-pound gorilla sitting in the corner of a natural history museum, the appropriately named Gigantopithecus was the largest ape that ever lived, not quite King Kong-sized but, at up to half a ton or so, much bigger than your average lowland gorilla. Or, at least, thats the way this prehistoric primate has been reconstructed; frustratingly, practically everything we know about Gigantopithecus is based on its scattered, fossilized teeth and jaws, which first came to the worlds attention when they were sold in Chinese apothecary shops in the first half of the 20th century. Paleontologists arent even sure how this colossus moved; the consensus is that it must have been a ponderous knuckle-walker, like modern gorillas, but a minority opinion holds that Gigantopithecus may have been capable of walking on its two hind feet. Another mysterious thing about Gigantopithecus is when, exactly, it lived. Most experts date this ape from Miocene to mid-Pleistocene eastern and southeastern Asia, about six million to one million years B.C., and it may have survived in small populations until as late as 200,000 or 300,000 years ago. Predictably, a small community of cryptozoologists insists that Gigantopithecus never went extinct, and persists in the present day, high up in the Himalayan Mountains, as the mythical Yeti, better known in the west as the Abominable Snowman! (Rest assured that no reputable scientists subscribe to this theory, which is supported by absolutely no compelling material or eyewitness evidence.) As fearsome as it must have looked, Gigantopithecus seems to have been mostly herbivorouswe can infer from its teeth and jaws that this primate subsisted on fruits, nuts, shoots and, just possibly, the occasional small, quivering mammal or lizard. (The presence of an unusual number of cavities in Gigantopithecus teeth also points to a possible diet of bamboo, much like that of a modern Panda Bear.) Given its size when fully grown, an adult Gigantopithecus would not have been an active target of predation, though the same cant be said for sick, juvenile or aged individuals, which figured on the lunch menu of various tigers, crocodiles and hyenas. Gigantopithecus comprises three separate species. The first and largest, G. blacki, lived in southeastern Asia starting in the middle Pleistocene epoch and shared its territory, toward the end of its existence, with various populations of Homo erectus, the immediate precursor of Homo sapiens. The second, G. bilaspurensis, dates to six million years ago, during the Miocene epoch, about the same early time frame as the oddly named G. giganteus, which was only about half the size of its G. blacki cousin.

Last Mohicans Essay Example

Last Mohicans Essay Example Last Mohicans Essay Last Mohicans Essay Essay Topic: Last Of the Mohicans The Heart Goes Last Plus scenes from the movie didnt match up properly with what Cooper had written. In the beginning of the novel the character David Gamut is introduced. He was a wandering young man in the forest who loved poetry. David was attempting to carry Christianity to the frontier through the power of his song. He was totally out of place in the wilderness, plus he was a subject of Hawkers mockery. He eventually became close with Hawker and helped him throughout their Journey. But for some reason he was of no relevance in the movie. David Gamut is a ghost to Michael Duns movie, he simply doesnt exist. Plus in the movie the eldest daughter Cord Munroe was castes and portrayed far differently than what Cooper had described. Cooper described his character Cord Munroe as an interracial child with an African American background. That is why they referred to her as the dark one. But in the movie she was played by an actress who was Caucasian, and the only thing that was dark about her was her hair. Cooper also made Corals love interest Uncaps, who is the full blood Monica son of Chicagoan. But the relationship ended tragically and there would have been no way possible for t to occur. Due to Cord and Uncaps being both of different nationalities. In Michael Duns movie Cord and Uncaps relationship didnt exist. The character Cord was played by the Caucasian actress Madeline Stows. She ended up falling in love with the storys hero Hawker. Both characters got to be together in the end of the movie. This is dramatically different than what Cooper had in his novel. Both Hawker and Cord could be together in Duns movie because it created no controversy over race. On another note, the movie showed that the younger sister Alice Munroe was in love tit Uncaps. When Magma was given Alice in the end of the movie to bare children with it sends Uncaps into a rage. As he tried to rescue Alice he was killed by Magma and thrown off the cliffs. Alice then commits suicide by slowly falling back off the mountain after her deceased love Uncaps. In comparison the novel and movie are wildly different. Characters in the movie are entirely different than what Cooper had created. They also are engaging in complete opposite roles. Important details in the novel made the story truer to its time. I suppose changing those important aspects of he novel made the movie more interesting, more action packed and to the point. Leave the storyline with a somewhat happy ending. In Coopers novel Hawkers relationship with the Musicians isnt as friendly as it is in the movie. In the movie when Hawker and the Musicians are introduced they act as if one another is family. Hawker refers to Chicagoan as his father and Uncaps his brother. Chicagoan Hawker and his adoptive family are close friends with Englishmen in the colony. They actually go to a gentlemans cabin that night and have dinner with this British colony family. Everyone interacts with one another very closely. Even the natives are eating dinner at the table and playing with the Englishmen son. In the novel Hawker doesnt share that same type of relationship with the Musicians, also continuously talks about how he is not of Indian blood. In the book he even states l am not a prejudiced man, nor one who vaunts himself on his natural privileges, though the worst enemy I have on earth, and he is an Iroquois, adrenal deny that I am genuine white . Hawkers adoptive background breeds both productive alliances and disturbingly racist convictions. He shows how the history and cultures of the people of the frontier influence one another. Regardless of the divide that the English, French etc want to create, everyone is going to somewhat intermix. But Hawker has a confliction wanting to state that he is white and has much more privileges than his friend. Yet he feels more comfortable in the wilderness with the native people. Hawker demonstrates that hes almost obsessed with proving hes not a native. Throughout Coopers novel he constantly brings up his white background to prove it. Scenes throughout Michaels Duns movie are so completely efferent than Coopers novel. In the beginning of the movie Hawker is hunting in the woods with the Musicians. Hawker shoots the deer with his long rifle with no worries at all. However, in Coopers story the Musicians keep Hawker from shooting the deer. They feared he would give up their location to their enemies so Uncaps kills the animal with a bow and arrow and finishes it off with a knife. Another part of Duns 1997 movie was definitely portrayed much different Coopers novel. Uncaps went to the tribal grounds of Magma to rescue Cord and the others. Demanded ends p letting Uncaps and Hawker go, but Magma takes Cord. Uncaps is so enraged he goes back to rescue Cord. When a native of Magma tribe gets impatient he stabs Cord in the heart. Uncaps and Magma battle it out but Magma slips off of the side of the mountain. As he slips Magma grasp hold of some trees and holds on for his life. But Hawker shoots him, and he falls back into his ultimate death. I truly believe both pieces of work were brilliant and beautiful. Yet it was difficult to understand Coopers novel, and was very lengthy. He emphasized a lot on the battles and showed the influences that all the people in the colony had on one another. But Dunn brought a lot of interesting elements to The Last Monica story. The battles were very realistic and thrilling. He showed that although the people in the colonies had issues there were some bonds and strong relationships among the different races. I personally would had never watched or read the book by choice myself. But I am glad I got to read, and watch two exceptional pieces of work. Although Dunn didnt tell the same story as Cooper, it was a very well made movie.

Cationic Antimicrobial Peptides in Humans

Cationic Antimicrobial Peptides in Humans Antimicrobial peptides Introduction Cationic antimicrobial peptides (AMPs) are gene-encoded peptides of the host defence system made up of 12-50 amino acids, with at least 2 positive charges conferred by lysine and arginine residues and about 50% hydrophobic amino acids (Hancock and Scott 2000). They are produced from gene transcription and ribosomal translation and often, further proteolytically processed (Zhoa 2003). The peptides are folded so that non-polar amino acid side-chains form a hydrophobic face and polar, positively charged residues form a hydrophilic face (Robert and Hancock 1997). Expression of antimicrobial peptides can be constitutive or inducible by infectious or inflammatory stimuli like cytokines, bacteria and lipopolysaccharides (LPS) (Cunliffe and Mahida 2004). They have diverse structures to effectively kill a wide range of microbes at prone sites e. g the skin and lungs, and in secretions such as sweat and saliva (Yeaman and Yount 2004; Santamaria 2005). Many mammalian antimicrobial peptides rouse the host’s innate immune system (Jenssen et al 2006) instead of directly killing the host. Peptides which are found in living organisms from bacteria to plants, insects, fish, amphibians to mammals including humans (Kamysz 2005) are recorded in numerous existing databases e. g. AMSDb (Eukaryotic peptides) (Tossi and Sandri 2002), BAPDb (bacterial peptides), ANTIMIC (natural antimicrobial peptides) (Brahmachary et al 2004) and APPDb. Currently, 1831 peptides are hosted by the Antimicrobial peptide database with 99 antiviral, 453 antifungal, 100 anticancer and 1179 antibacterial peptides (The Antimicrobial Peptide database 2010). In humans, antimicrobial peptides are produced by granulocytes, macrophages and most epithelial and endothelial cells. They boost the immune system, have anti-neoplastic properties and help in regulating cell signalling and multiplication. Amphibian AMPs have been discovered from the skin of frogs from families ra nging from Iomedusa, Pipidae, Hyperoliidae, Ranidae, Hylidae, Discoglossidae, Agalychnis and Litoria. The structure of these peptides as unravelled by CD spectroscopy, NMR spectroscopy and molecular modeling (Suh et al 1996) have been found to be generally 10-46 amino acid residues long (Rollins-Smith et al 2005), mostly linear and simple-structured, (Conlon et al 2004) the majority being hydrophobic, cationic and possessing an amphipathic a-helix in nature. Following production, they are stored in the granular glands (poison glands) of skin dermal layer to be secreted in response to injury (Bovbjerg 1963), or as defence against pathogenic bacteria, fungi, viruses and parasites. Biologically active molecules including antimicrobial peptides are produced as large proteins harbouring a signal and an acidic propiece which get cut off to give an active peptide prior to or at secretion from the poison glands (Amiche et al 1999). Cationic peptides are also expressed in the gastric mucosa cells and in the intestinal tract (Kamysz 2005). The best-known peptides isolated from frogs are caeruleins, tachykinins, bradykinins, thyrotropin- releasing hormone (Barra and Simmaco 1995), brevinins, esculentins, magainins, ranatuerins and temporins (Conlon et al 2004). In the past, peptides were extracted using solvents like methanol or acid from the skins of amphibians after sun-drying but with concomitant dwindling of many frog species, other alternative techniques have emerged, one of which comprises stimulating the frog using mild electricity and collecting the skin secretion; 2-4 weeks after, the secretion can be re-collected after replenishment of the glands (Barra and Simmaco 1995). Large amounts of small peptides and their analogues which are resistant to protease cleavage and contain D-amino acids can be chemically synthesised while larger peptides can be expressed in a prokaryotic host from cloned cDNAs coding for a fusion protein (Piers et al 1993). An efficient means of producing therapeutic peptides in transgenic mice red blood cells has been explained by Sharma et al (1994) whereby the required peptide is collected from proteolytic cleavage from the fusion protein where the peptide is at the C-terminal end of human a-globin.

ALTERNATIVE DISPUTE RESOLUTION (easy work) Research Paper

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The main reason couples divorce Essay Example | Topics and Well Written Essays - 1500 words

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Romantic music in Renaissance painting Essay Example | Topics and Well Written Essays - 1000 words

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